In total, 137 patients with PCLs diagnosed in our hospital in 2008-2017 were retrospectively reviewed. To evaluate the frequency, demographic data, and clinical characteristics of different subtypes of PCLs in a tertiary care university hospital. The epidemiological and clinical data of PCLs in Thailand are lacking. Primary cutaneous lymphomas (PCLs) refer to cutaneous lymphomas that primarily develop in the skin with no evidence of extracutaneous disease at the time of diagnosis. Our observations suggest that cutaneous T-cell lymphoma should be included in the differential diagnoses of exfoliative dermatitis, even those progressing in a chronic pattern and/or with few or no pruritus. This case report provides the first evidence of clinical, histopathological, immunophenotypic features, electron microscopy findings and molecular analysis of a cutaneous epitheliotropic T-cell lymphoma (mycosis fungoides) in a donkey. Transmission electronic microscopy showed multiple lymphocytes with convoluted or cerebriform nuclei. Molecular clonality PCR (PARR) was performed using equine TRG primers this revealed a clonal rearrangement in a heavy polyclonal background. Immunohistochemical staining revealed uniform labelling of the neoplastic cells for CD3, and lack of expression of CD20 (a B cell lineage associated marker). These findings are consistent with cutaneous epitheliotropic lymphoma. Clusters of neoplastic cells were present within the epidermis forming Pautrier “microabscesses”. Histological findings were characterised by interface dermatitis with massive exocytosis in the epidermis of a homogenous population of lymphoid cells showing atypia. Protein electrophoresis showed moderate polyclonal hypergammaglobulinemia. The biochemistry panel showed mild hyperproteinemia with albumin within the normal range. The complete blood count and blood smear evaluation revealed mild normocytic normochromic anemia. Ectoparasites and dermatophytes were ruled out. All mucocutaneous junctions except the hoofs were affected. Dermatological examination revealed severe generalised alopecic and exfoliative dermatitis, occasionally eroded, with high number of large, thin, greyish scales. Otherwise, the animal was clinically healthy, though mildly underweight. In the present paper, we report a case of mycosis fungoides diagnosed in a donkey whose diagnosis was based on clinical, histological and immunohistochemical aspects and a positive TRG clonality test.Ī twenty-five-year-old donkey gelding was referred with a mildly pruritic, generalised and severe exfoliative dermatosis. Differentiation of malignant versus benign proliferation of lymphocytes is crucial in ambiguous cases T-cell receptor gamma (TRG) molecular clonality should be tested. The standard diagnosis is based on clinical, morphological and immunophenotypic data. Cutaneous epitheliotropic T-cell lymphoma is a malignant tumour of the skin already reported in humans, dogs, cats, horses, and other species, but not previously in donkeys.
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